Stark's Buffer

Posted on May 18th, 2016 by UNM CC

M.Fero — 1/17/2001

Final Concentration

  • 5x SSC
  • 25 mM NaPO4, pH6.5
  • 5x Denhardt's Solution
  • 0.25 mg/mL Torula RNA
  • 50% (v/v) Formamide

Stock Solutions

  • 20x SSC: 3 M NaCl, 0.3 M NaCitrate, pH7.0
  • 50x Denhardt's: 1% (w/v) BSA, 1% (w/v) PVP-40, 1% (w/v) Ficoll-400
  • 10 mg/mL Torula RNA (boil to dissolve)
  • 1 M NaPO4, pH 6.5

Preparation

To make 1 L. of Stark's Buffer mix:

  • 250 mL 20x SSC
  • 25 mL 1M NaPO4, pH6.5
  • 100 mL 50x Denhardt's solution
  • 25 mL 10 mg/mL Torula RNA (boil just prior to adding)
  • 122.5 mL H2O
  • 500 mL Formamide

Tags: Fero-DNA

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