Posted on July 15th, 2016 by UNM CC
(M. Fero, courtesy of the Porter lab)
Also see Wei-Ming and Emily's protocol This reference link is broken.
Stains (change after every 3rd use)
Harris hematoxylin: 1X stock. Anatech Lmtd. Cat #842. (616) 964-6450
Gill's Hematoxylin: See recipe.
Eosin: 1x stock. Anatech Cat #837.
Solutions (make fresh each day)
Acid alcohol: 75% EtOH + 1/2500 v/v concentrated HCl (156 mL 95% EtOH + 44 mL H2O + 80 µL HCl = 200 mL)
Ammonia Solution: 0.084% (w/v) NH4OH (200 mL H2O + 0.6 mL 28% w/v NH4OH stock).
Xylene x2 (Make at least 1 fresh)
100% EtOH (Make at least 1 fresh)
95% EtOH x2 (make both fresh)
80% EtOH x1 (make fresh = 168 mL 95% EtOH + 32 mL H2O)
(Note: Unless otherwise specified, slides should be dipped 10 times in each solution)
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