Posted on August 1st, 2016 by UNM CC
M. Fero — 3/24/04 — adapted from P. Soriano
2% Paraformaldehyde (in PBS)
5 mM potassium ferricyanide,
5 mM potassium ferrocyanide,
2 mM MgCI,
0.01% sodium deoxycholate
0.02% Nonidet P-40 (NP-40)
40 mg/mL X-Gal (5 -Bromo-4-chloro-3-indolyl-beta-D-galactopyranoside)
store at -20°C in small aliquots.
X-gal Mix (make fresh just before use)
10 mL Staining solution
0.25 mL X-gal Stock (final = 1 mg/mL).
Be sure that X-Gal is well dissolved before use otherwise crystals may form in the staining solution
You must be logged-in to the site to post a comment.