Equipment

The IVIS Spectrum In Vivo Optical Imaging System

A 3D rendering from the IVIS Spectrum

The IVIS Spectrum provides both bioluminescence and fluorescence imaging of live mice using epi- or trans-illumination.   Up to 5 mice can be imaged at once. Spectral unmixing allows imaging of multiple fluorescent probes in the same animal and can subtract autofluorescence signal.  Bioluminescence or Fluorescence Tomography software allows for 3D reconstruction of tumors.non-invasive tumor imagings

 

  • heated platform and built-in anesthesia unit
  • high throughput: up to 5 mice (26 cm FOV)
  • high resolution: 20 μm resolution (3.9 cm FOV)
  • fluorescent and/or bioluminescent detection
  • Epi- or transillumination
  • 28 filters spanning 430 nm to 840 nm easily distinguishes multiple reporters
  • spectral unmixing
  • 3D imaging

The IVIS can detect deep tissue tumors of 1 X 10cells and subcutaneous cancer cells down to 50 cells.

To learn more about the instrument capabilities, contact the Shared Resource personnel or visit the following site: http://www.perkinelmer.com/product/ivis-instrument-spectrum-120v-andor-c-124262

Annual Lab fee: $1100/lab

Training is provided by the Animal Models Shared Resource.

The Faxitron MultiRad Irradiator

The MultiRad 225 Irradiator is a compact X-Ray Irradiation System, which is a safer alternative to radioisotope irradiators. It delivers a broad range of doses with a uniform beam profile.  Applications include irradiation of feeder cells, oncology research, sterilization studies, and small animal myeloablation/ bone marrow transplant studies. Up to 12 mice can be irradiated at once using a plexiglass carousel.

  • self-shielded for safety
  • uses and x-ray tube to create ionizing radiation (no radioactive source)
  • can irradiate:
    • flasks of cells for feeder cell preparation
    • mice for bone marrow ablation or irradiation studies
  • 12 mice can be irradiated at a time

To learn more about the instrument capabilities, contact the Shared Resource personnel or visit the following site: http://www.faxitron.com/life-sciences-ndt/products/multirad.html

Annual fee: $875/lab

Training by an Animal Models Shared Resource specialist is required before unassisted use can be scheduled.