Equipment

The IVIS Spectrum In Vivo Optical Imaging System

A 3D rendering from the IVIS Spectrum

The IVIS Spectrum provides both bioluminescence and fluorescence imaging of live mice using epi- or trans-illumination.   Up to 5 mice can be imaged at once. Spectral unmixing allows imaging of multiple fluorescent probes in the same animal and can subtract autofluorescence signal.  Bioluminescence or Fluorescence Tomography software allows for 3D reconstruction of tumors.non-invasive tumor imagings

  • heated platform and built-in anesthesia unit
  • high throughput: up to 5 mice (26 cm FOV)
  • high resolution: 20 μm resolution (3.9 cm FOV)
  • fluorescent and/or bioluminescent detection
  • Epi- or transillumination
  • 28 filters spanning 430 nm to 840 nm easily distinguishes multiple reporters
  • spectral unmixing
  • 3D imaging

The IVIS can detect deep tissue tumors of 1 X 10cells and subcutaneous cancer cells down to 50 cells.

To learn more about the instrument capabilities, contact the Shared Resource personnel or visit the following site: http://www.perkinelmer.com/product/ivis-instrument-spectrum-120v-andor-c-124262

Annual Lab fee: $1,500/lab

Training is provided by the Animal Models Shared Resource.

The Faxitron MultiRad Irradiator

The MultiRad 225 Irradiator is a compact X-Ray Irradiation System, which is a safer alternative to radioisotope irradiators. It delivers a broad range of doses with a uniform beam profile.  Applications include irradiation of feeder cells, oncology research, sterilization studies, and small animal myeloablation/ bone marrow transplant studies. Up to 12 mice can be irradiated at once using a plexiglass carousel.

  • self-shielded for safety
  • uses and x-ray tube to create ionizing radiation (no radioactive source)
  • can irradiate:
    • flasks of cells for feeder cell preparation
    • mice for bone marrow ablation or irradiation studies
  • 12 mice can be irradiated at a time

To learn more about the instrument capabilities, contact the Shared Resource personnel or visit the following site: http://www.faxitron.com/life-sciences-ndt/products/multirad.html

Annual fee: $1,000/lab

Training by an Animal Models Shared Resource specialist is required before unassisted use can be scheduled.

Assisted use for the IVIS or Multirad Irradiator can be scheduled for $90/hour, no training or annual fee required.

Animal Physiology Equipment

The Abaxis Vetscan HM5 Hematology Analyzer

The VetScan HM5 is a Five-Part Differential Hematology Analyzer for use in animal studies. It is fully-automated and displays a comprehensive 22-parameter complete blood count (CBC), including direct eosinophil counts and eosinpophil percentages with cellular histograms on an easy-to-read touch-screen. VetScan HM5 has 15 preloaded programs to perform five-part differential analysis (two subtypes of granulocytes, eosinophils, neutrophils and basophils) on cat, dog, horse, cow, alpaca and llama and three-part analysis on on mouse, rabbit, rat, ferret, pig, goat, monkey, sheep and guinea pig. Minimum sample size is 50 μL (five-part differential) and 25 μL (three-part differential). It is highly accurate and gives reproducible results comparable to reference lab analyzers. The HM5 has also been validated for use with peritoneal and synovial fluids.

Training is offered for a one-time fee of $206.00 per user.

Labs may also elect to have Animal Models Shared Resource personnel run samples for a fixed fee per sample. If you are interested in this option, please contact Irina Lagutina.

Unassisted use: Labs will be charged a reagent fee of $8/sample for use of the Vetscan HM5.

Assisted use: Labs will be charged $13/sample for samples run by the Animal Models Core.

The Abaxis Vetscan VS2 Analyzer

Vetscan VS2 is a small animal chemistry, electrolyte, immunoassay and blood gas analyzer that performs accurate analysis from just two drops of whole blood, serum or plasma. 13 VetScan profiles are available from Abaxis including an electrolyte, kidney plus, mammalian liver, and T4/cholesterol profile. Profiles are packaged as single-use plastic rotors (one rotor/sample). Each reagent rotor contains a diluent and all necessary components to perform a complete multi-chemistry blood analysis. Rotors can be purchased by the users directly from Abaxis:

The core has two VS2, which can be booked separately and used simultaneously.

Before using VS2 independently, users have to be trained by core personal.

Vetscan VS2 users will be charged an annual fee of $206.00 per lab for unassisted use by trained personnel.

Labs may also elect to have Animal Models Shared Resource personnel perform analyses for them for a fixed fee per sample. If you are interested in this option, please contact Mara Steinkamp or Irina Lagutina.