Services

Animal Models Consultation

To discuss a new project, please contact the Animal Models faculty director, Mara Steinkamp, Ph.D. or initiate a consultation on CrossLab.

Services include:

  • Assistance with animal model selection, study planning, and design
  • Study interpretation
  • Assistance with manuscripts and letters of support for grant preparation
  • Training
  • Animal protocol assistance
  • Planning/Referral for animal modeling techniques/training

Animal Pathology Services

To discuss a new project, please contact Dr. Donna Kusewitt, DVM, PhD, ACVP or initiate a pathology consultation request on CrossLab.

Services include:

  • Assistance with animal model selection and study design
  • Necropsy
  • Descriptive and semi-quantitative histopathology
  • Referrals for slide preparation and immunohistochemistry
  • Morphometry
  • Photomicroscopy
  • Serum chemistry and hematology
  • Training in necropsy and tissue collection techniques
  • Study interpretation
  • Assistance with manuscript and grant preparation

NOD scid gamma (NSG) immunodeficient mice

In-house bred NOD scid gamma (NSG) immunodeficient mice

We support a breeding colony of NOD scid gamma (NSG) mice (https://www.jax.org/strain/005557) for use in xenograft models, particularly patient-derived xenograft (PDX) models, and "humanized" mouse models.

Requests for NSG mice can be made through CrossLab.

Animal Models Technical Services

Core personnel can provide technical help with specialized surgical procedures, drug delivery for therapeutic trials, tumor monitoring and measurement, personnel training, instrument operation, and data analysis /troubleshooting. We also offer complete xenograft services. The client provides the cells, and the Shared Resource personnel will introduce the xenograft into a suitable mouse host*. Xenograft implantation includes one cell line or sample implanted in up to 5 mice (one cage).

*NSG mice can be purchased from the Core. Other host mice can be purchased from outside sources.

Basic Xenograft Implantation

Animal Models personnel will inject cell suspensions I.V., I.P., or SubQ into mice.

Services include:

  1. Tumor cell inoculation
  2. 1 week post-inoculation monitoring
  3. Necropsy and fixation/freezing of tumor and tissues of interest

Complex Xenograft Implantation

Animal Models personnel will implant cells or tumor tissue into mice.

Services include:

  1. Surgical engraftment of tumor tissue
  2. 1 week post-engraftment monitoring
  3. Necropsy and fixation/freezing of tumor and tissues of interest

Complex xenograft implantation includes one tumor sample implanted in up to 5 mice (one cage).

Tumor Measurement

Tumors can be measured on a predetermined schedule based on the experimental design. Lab staff will be updated weekly.

Tumor Monitoring

Mice can be monitored for tumor growth and general health. Lab staff will be updated weekly.