Items Tagged ‘trizol’

May 18, 2016

TRIzol Prep

By UNM CCC robin

v.2 M. Fero 6/1/06 Procedure Homogenize cells (10 million) or tissue (50-100 mg) in 1 mL TRIzol Reagent (e.g. scrape and pass through 30G needle, dounce homogenize and pass through needle, or use a homogenizer) and transfer to a 1.5 mL tube. Optional – Spin at 12k g for 10′ at r.t. to pellet debris. […]

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Tags: Fero-RNA, trizol