M.Fero — 1/17/2001
Final Concentration
- 5x SSC
- 25 mM NaPO4, pH6.5
- 5x Denhardt's Solution
- 0.25 mg/mL Torula RNA
- 50% (v/v) Formamide
Stock Solutions
- 20x SSC: 3 M NaCl, 0.3 M NaCitrate, pH7.0
- 50x Denhardt's: 1% (w/v) BSA, 1% (w/v) PVP-40, 1% (w/v) Ficoll-400
- 10 mg/mL Torula RNA (boil to dissolve)
- 1 M NaPO4, pH 6.5
Preparation
To make 1 L. of Stark's Buffer mix:
- 250 mL 20x SSC
- 25 mL 1M NaPO4, pH6.5
- 100 mL 50x Denhardt's solution
- 25 mL 10 mg/mL Torula RNA (boil just prior to adding)
- 122.5 mL H2O
- 500 mL Formamide
Tags: Fero-DNA, Fero-Lab-Protocols