M.Fero — 1/17/2001

Final Concentration

• 5x SSC
• 25 mM NaPO4, pH6.5
• 5x Denhardt's Solution
• 0.25 mg/mL Torula RNA
• 50% (v/v) Formamide

Stock Solutions

• 20x SSC: 3 M NaCl, 0.3 M NaCitrate, pH7.0
• 50x Denhardt's: 1% (w/v) BSA, 1% (w/v) PVP-40, 1% (w/v) Ficoll-400
• 10 mg/mL Torula RNA (boil to dissolve)
• 1 M NaPO4, pH 6.5

Preparation

To make 1 L. of Stark's Buffer mix:

• 250 mL 20x SSC
• 25 mL 1M NaPO4, pH6.5
• 100 mL 50x Denhardt's solution
• 25 mL 10 mg/mL Torula RNA (boil just prior to adding)
• 122.5 mL H2O
• 500 mL Formamide

Tags: Fero-DNA, Fero-Lab-Protocols