July 25, 2018
By UNM CCC 
(M. Fero — 1/4/95, with thanks to P. Soriano) MATERIALS Glassware: All hand washed with no soap. Gelatin (Sigma G-1393) Diluted to 0.1% in H2O, + 1 drop of media to colorize Plastic pipets, P100 tissue culture dishes and 24 well multiwell dishes. Trypsin (Gibco 25200-023) BioRad cuvettes (0.4 cm, electrode gap 50, Cat# 165.2088) […]
Tags: Fero-Cell
August 1, 2016
By UNM CCC
Determine the final volume (Vf): How much to you want to make? Determine the dilution factor (d.f.). User either… d.f. = Cs / Cf i.e. the ratio of starting concentration / final concentration: d.f. = the fold concentration (e.g. 20x) of the starting material (if the final concentration is 1x) Calculate the starting volume (Vs): […]
Tags: Fero-Formulae
August 1, 2016
By UNM CCC
A common issue in designing a clinical or animal study is to determine the number of subjects required. Power calculations provide an estimate of the number of study subjects that will be necessary to be able detect a difference between experimental and control subjects. The numbers required will depend on the magnitude of the difference […]
Tags: Fero-Formulae
August 1, 2016
By UNM CCC
For your convenience, you can download the manual here: DirectPCR Tail DNA Preps
Tags: Fero-Manuals
August 1, 2016
By UNM CCC
For your convenience, you can download the manual here: ImageJ-guide
Tags: Fero-Manuals
August 1, 2016
By UNM CCC
For your convenience, you can download the manual here: HB6_rotor_specs
Tags: Fero-Manuals
August 1, 2016
By UNM CCC
For your convenience, you can download the manual here: RNeasy_Mini_Handbook
Tags: Fero-Manuals
August 1, 2016
By UNM CCC
For your convenience, you can download the manual here: BioRad_Bradford
Tags: Fero-Manuals
August 1, 2016
By UNM CCC
For your convenience, you can download the manual: TRIzol_Reagent
Tags: Fero-Manuals
August 1, 2016
Fluorescent in Situ Hybridization
By UNM CCC
M. Fero 4/6/2006 (courtesy of Katie Rudd, Trask lab) Materials 20 ng BAC DNA clone grown o.n. in 5 mL LB + chloramphenicol Autogen reagents Slide warmer Organics: MeOH, EtOH, formamide Fixative (3:1 MeOH:glacial HOAc) Biotin (or digoxigenin) nick-translation Kit (Invitrogen) 20x SSC (3 M NaCl, 0.3 M NaCitrate, pH 7.0) TE, pH8 CoT DNA […]
Tags: Fero-Histology
August 1, 2016
Anti-GFP/BrdU In Situ Immunostaining
By UNM CCC
<R. Gu 4/6/2005 Materials 24 well dishes Millicell 0.4 µm inserts (Millipore PICM01250) – coated with fibronectin Culture medium PBS Fixative: 4% paraformaldehyde in PBS (pH 7.6) Rabbit anti-GFP (Molecular Probes, A-11122) FITC-goat anti-rabbit IgG (Jackson ImmunoResearch, 111-096-006) Biotinylated anti-BrdU (AbCam, ab2284-125) Streptavidin-Alexa Flour 555 (Invitrogen/Molecular Probes, S21381) Goat serum Horse serum BSA 2N HCl […]
Tags: Fero-Histology
August 1, 2016
Immunostaining with Anti-Digoxigenin Alkaline Phosphatase
By UNM CCC
Modified from C.H. Lin 5/2005 Materials Anti-digoxigenin-AP conjugate (Roche #11093274910, 150U/200 uL, $168) Levamisole (Sigma #T1512, 2 g, $10) (blocks endogenous phosphatase activity) BCIP (Roche #11383221001, 3 mL, $49) NBT (Roche #11383213001, 3 mL, $48) Atlternate BCIP/NBT (Roche, #11697471001, 20 Tabs, makes 10 mL/Tab, $67) Blocking Reagent (Roche #11096176001, 50 g, $104, or 10x Block […]
Tags: Fero-Histology
August 1, 2016
Immunohistochemistry (IHC) of Thymus Frozen Sections
By UNM CCC
(courtesy of Farr lab, UWMC) Materials Cold Acetone (-20ºC) in Coplin jars PBS, BenchKote paper, plastic lids Anti-digoxigenin-HRP diluent: 1% BSA in PBS (store 10 mL aliquots of BSA at -20ºC) Anti-rat-digoxigenin diluent: 5% Milk powder, 10% mouse serum, 10% goat serum, 1% BSA in PBS Primary and secondary antibodies (this had a link that […]
Tags: Fero-Histology
