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A/B/C Random Amplification Protocol
Alcohol Precipitation of DNA
AML Sample Genomic DNA Preparation
Electroelution
Fluorometric Quantitation of RNA or DNA
Nanodrop
New Oligo in the Lab
Q-PCR
Southern Hybridazation
Stark’s Buffer
Tail DNA Preparation
384 Well Template
Amino-Allyl cDNA Labeling
Bioanalyzer Sample Preparation
mRNA Amplification with T7 RNA Polymerase
RNA Gel Electrophoresis
RNEasy Midi Protocol
TRIzol Prep
Histone Kinase Assay
p27 ELISA
Western Blot Protocol
Colony Hybridization
General Cloning Protocols
Large Scale Plasmid Preps: PEG method
Large Scale Plasmid Preps: Qiagen/Cesium Method
Ligation Optimization
Plasmid Miniprep
Preparation of Electrocompetent Bacteria
Transformation of Electrocompetent E. coli with [...]
96-Well Flow Setup Record
BrDU and PI Staining for Cell [...]
Counts of Fluorescently Stained Cells
Flow Cytometry of Fibroblast Nuclei for [...]
Flow Sorting Fibroblasts with GFP and [...]
Lineage Analysis of Blood
Simultaneous Cell Surface and BrdU staining [...]
Simultaneous Nuclear and Surface Immunostaining for [...]
Use of the B.D. FACS or [...]
Culturing Mouse Embryonic Fibroblasts
ES and STO cell culture
Harvesting Hematopoietic Cells from Mice
Purification of L-KS+ Marrow Cells
Simplified Calcium Phosphate Coprecipitation
Mouse Compete Blood Counts
Mouse p27 PCR Genotyping Using Gitschier [...]
Mouse Toe Numbering Scheme
Necropsy Form
Using the MPD
Xenogen Imager
Anti-GFP/BrdU In Situ Immunostaining
BrdU-Immunoperoxidase Staining of Paraffin Tissue Sections
Complete Mouse Necropsy
Fluorescent in Situ Hybridization
Hematoxylin and Eosin (H&E) Staining
Immunoflourescence of Cultured Cells
Immunohistochemistry (IHC) of Thymus Frozen Sections
Immunostaining with Anti-Digoxigenin Alkaline Phosphatase
Tissue Processing and Sectioning
Tissue Staining
X-gal Staining Procedure
BioRad Bradford Protein Assay
DirectPCR Tail DNA Preps
ImageJ User Guide
Qiagen RNEasy Mini
Sorvall HB6 Rotor Specs
TRIzol Reagent
Formulae
Power Curves
Simple Dilutions
