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DNA

  • A/B/C Random Amplification Protocol  
  • Alcohol Precipitation of DNA
  • AML Sample Genomic DNA Preparation
  • Electroelution
  • Fluorometric Quantitation of RNA or DNA
  • Nanodrop
  • New Oligo in the Lab
  • Q-PCR
  • Southern Hybridazation
  • Stark’s Buffer
  • Tail DNA Preparation

    • RNA

  • 384 Well Template
  • Amino-Allyl cDNA Labeling
  • Bioanalyzer Sample Preparation
  • mRNA Amplification with T7 RNA Polymerase
  • RNA Gel Electrophoresis
  • RNEasy Midi Protocol
  • TRIzol Prep

    • Protein

  • Histone Kinase Assay
  • p27 ELISA
  • Western Blot Protocol

    • Bacteria

  • Colony Hybridization
  • General Cloning Protocols
  • Large Scale Plasmid Preps: PEG method
  • Large Scale Plasmid Preps: Qiagen/Cesium Method
  • Ligation Optimization
  • Plasmid Miniprep
  • Preparation of Electrocompetent Bacteria
  • Transformation of Electrocompetent E. coli with Blue/White Selection

    • Flow Cytometry

  • 96-Well Flow Setup Record
  • BrDU and PI Staining for Cell Cycle Analysis
  • Counts of Fluorescently Stained Cells
  • Flow Cytometry of Fibroblast Nuclei for DNA Content
  • Flow Sorting Fibroblasts with GFP and P.I.
  • Lineage Analysis of Blood
  • Simultaneous Cell Surface and BrdU staining for Flow Cytometry
  • Simultaneous Nuclear and Surface Immunostaining for Flow Cytometry
  • Use of the B.D. FACS or Calibur Flow Cytometers

    • Cell Culture

  • Culturing Mouse Embryonic Fibroblasts
  • ES and STO cell culture
  • Harvesting Hematopoietic Cells from Mice
  • Purification of L-KS+ Marrow Cells
  • Simplified Calcium Phosphate Coprecipitation

    • Mice

  • Mouse Compete Blood Counts
  • Mouse p27 PCR Genotyping Using Gitschier Buffer
  • Mouse Toe Numbering Scheme
  • Necropsy Form
  • Tail DNA Preparation
  • Using the MPD
  • Xenogen Imager

    • Histology

  • Anti-GFP/BrdU In Situ Immunostaining
  • BrdU-Immunoperoxidase Staining of Paraffin Tissue Sections
  • Complete Mouse Necropsy
  • Fluorescent in Situ Hybridization
  • Hematoxylin and Eosin (H&E) Staining
  • Immunoflourescence of Cultured Cells
  • Immunohistochemistry (IHC) of Thymus Frozen Sections
  • Immunostaining with Anti-Digoxigenin Alkaline Phosphatase
  • Necropsy Form
  • Tissue Processing and Sectioning
  • Tissue Staining
  • X-gal Staining Procedure

    • Product Manuals

  • BioRad Bradford Protein Assay
  • DirectPCR Tail DNA Preps
  • ImageJ User Guide
  • Qiagen RNEasy Mini
  • Sorvall HB6 Rotor Specs
  • TRIzol Reagent

    • Useful Formulae

  • Formulae
  • Power Curves
  • Simple Dilutions
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