UNM Health
NCI Comprehensive Cancer Center

Protocols

Fero Lab
• Opportunities
• Publications
• Resources
• Protocols

Note: Depending on your system or browser the greek letter 'μ' (mu) may erroneously appear as an 'm'. This might make units such as microliters appear as mililiters. Please be cognizant of this and let us know if something appears wrong.

Search Protocols

DNA

  • A/B/C Random Amplification Protocol  
  • Alcohol Precipitation of DNA
  • AML Sample Genomic DNA Preparation
  • Electroelution
  • Fluorometric Quantitation of RNA or DNA
  • Nanodrop
  • New Oligo in the Lab
  • Q-PCR
  • Southern Hybridazation
  • Stark’s Buffer
  • Tail DNA Preparation

    • RNA

  • 384 Well Template
  • Amino-Allyl cDNA Labeling
  • Bioanalyzer Sample Preparation
  • mRNA Amplification with T7 RNA Polymerase
  • RNA Gel Electrophoresis
  • RNEasy Midi Protocol
  • TRIzol Prep

    • Protein

  • Histone Kinase Assay
  • p27 ELISA
  • Western Blot Protocol

    • Bacteria

  • Colony Hybridization
  • General Cloning Protocols
  • Large Scale Plasmid Preps: PEG method
  • Large Scale Plasmid Preps: Qiagen/Cesium Method
  • Ligation Optimization
  • Plasmid Miniprep
  • Preparation of Electrocompetent Bacteria
  • Transformation of Electrocompetent E. coli with Blue/White Selection

    • Flow Cytometry

  • 96-Well Flow Setup Record
  • BrDU and PI Staining for Cell Cycle Analysis
  • Counts of Fluorescently Stained Cells
  • Flow Cytometry of Fibroblast Nuclei for DNA Content
  • Flow Sorting Fibroblasts with GFP and P.I.
  • Lineage Analysis of Blood
  • Simultaneous Cell Surface and BrdU staining for Flow Cytometry
  • Simultaneous Nuclear and Surface Immunostaining for Flow Cytometry
  • Use of the B.D. FACS or Calibur Flow Cytometers

    • Cell Culture

  • Culturing Mouse Embryonic Fibroblasts
  • ES and STO cell culture
  • Harvesting Hematopoietic Cells from Mice
  • Purification of L-KS+ Marrow Cells
  • Simplified Calcium Phosphate Coprecipitation

    • Mice

  • Mouse Compete Blood Counts
  • Mouse p27 PCR Genotyping Using Gitschier Buffer
  • Mouse Toe Numbering Scheme
  • Necropsy Form
  • Tail DNA Preparation
  • Using the MPD
  • Xenogen Imager

    • Histology

  • Anti-GFP/BrdU In Situ Immunostaining
  • BrdU-Immunoperoxidase Staining of Paraffin Tissue Sections
  • Complete Mouse Necropsy
  • Fluorescent in Situ Hybridization
  • Hematoxylin and Eosin (H&E) Staining
  • Immunoflourescence of Cultured Cells
  • Immunohistochemistry (IHC) of Thymus Frozen Sections
  • Immunostaining with Anti-Digoxigenin Alkaline Phosphatase
  • Necropsy Form
  • Tissue Processing and Sectioning
  • Tissue Staining
  • X-gal Staining Procedure

    • Product Manuals

  • BioRad Bradford Protein Assay
  • DirectPCR Tail DNA Preps
  • ImageJ User Guide
  • Qiagen RNEasy Mini
  • Sorvall HB6 Rotor Specs
  • TRIzol Reagent

    • Useful Formulae

  • Formulae
  • Power Curves
  • Simple Dilutions
    • © 2020 UNM Health Sciences Center

    version 3.3.6.2.3.2
    Page loaded in 0.648 seconds