To discuss a new project, please contact the Animal Models faculty director, Mara Steinkamp, Ph.D. or initiate a consultation on CrossLab.
In collaboration with the Flow Cytometry Shared Resource, we have established two humanized mouse models at UNM:
Immunocompromised mice are engrafted with cord-blood derived CD34+ cells (single donor or multiple donor) to “humanize” the mouse immune system. Peripheral blood is assessed for human B cells, T cells, and myeloid cells 12 weeks post-engraftment to ensure humanization. These specialized mice are engrafted upon request.
Please contact Irina Lagutina for more information.
To discuss a new project, please contact Dr. Donna Kusewitt, DVM, PhD, ACVP or initiate a pathology consultation request on CrossLab.
We support a breeding colony of NOD scid gamma (NSG) mice (https://www.jax.org/strain/005557) for use in xenograft models, particularly patient-derived xenograft (PDX) models, and "humanized" mouse models.
We also carry NOD.Cg-KitW-41J Tyr + Prkdcscid Il2rgtm1Wjl/ThomJ (NBSGW) immunocompromised mice that can be engrafted with human immune cells without the need for pre-engraftment irradiation.
Requests for NSG mice can be made through CrossLab.
Core personnel can provide technical help with specialized surgical procedures, drug delivery for therapeutic trials, tumor monitoring and measurement, personnel training, instrument operation, and data analysis /troubleshooting. We also offer complete xenograft services. The client provides the cells, and the Shared Resource personnel will introduce the xenograft into a suitable mouse host*. Xenograft implantation includes one cell line or sample implanted in up to 5 mice (one cage).
*NSG mice can be purchased from the Core. Other host mice can be purchased from outside sources.
Animal Models personnel will inject cell suspensions I.V., I.P., or SubQ into mice.
Animal Models personnel will implant cells or tumor tissue into mice.
Complex xenograft implantation includes one tumor sample implanted in up to 5 mice (one cage).
Staff can administer treatments to mice via oral gavage, IM, IP or IV injection following investigator-specified dosing regimens. Staff are available to treat mice as required on weekdays and/or weekends with adequate lead time.
Tumors can be measured on a predetermined schedule based on the experimental design. Lab staff will be updated weekly.
Mice can be monitored for tumor growth and general health. Staff are available to monitor mice as required on weekdays and/or weekends with adequate lead time.