Fluorescence Microscopy & Cell Imaging Shared Resource

Acknowledgment

To acknowledge use of this shared resource, please include the following in your publications: This research was partially supported by UNM Comprehensive Cancer Center Support Grant NCI P30CA118100 and made use of the Fluorescence Microscopy and Cell Imaging shared resource.

Microscopy

Microscopy is at the heart of understanding tissue architecture, cell structure and dynamics, as well as molecular function. Fluorescence microscopy is routinely used to determine spatial and topological information about cells and tissues. Sophisticated laser scanning microscopic instrumentation, ultra sensitive digital cameras and specialized fluorescence probes make it possible to visualize cellular events in real time down to the molecular level.

Location: Cancer Research Facility (CRF), Rooms 212, 216, 218, 224, 226

The Fluorescence Microscopy Shared Resource:

The Fluorescence Microscopy and Cell Imaging Shared Resource advances cancer research excellence by making available state-of-the-art instrumentation and software for quantitative, multi-scale, and multi-modal fluorescence imaging. 

I. Aids basic and physician researchers to image samples and publish high profile articles that:

• Elucidate cell and molecular mechanisms of cancer, immunologic, infectious, metabolic, neurologic and vascular diseases
• Evaluate therapeutic efficacy in cells and patient samples
• Test new nanotechnologies in cell based assays
• Quantitatively measure changes in tissue morphology and pathology

II. Provides UNM researchers access to state-of-the art instrumentation for multiple fluorescence and transmitted light microscopy techniques:

• Laser scanning single and multi-photon microscopes and hyperspectral imaging systems enable simultaneous visualization and quantification of histochemical stains and fluorescent labels
• Total Internal Reflection Fluorescence (TIRF), Single Particle Tracking (SPT) and Single Molecule Localization Microscopy (SMLM) applications to quantify dynamic live-cell events and achieve resolution below the diffraction limit of light
• Ratiometric imaging allows quantification of real time changes in calcium and other ion concentrations in response to cell signaling 

III. Has resource staff who offer investigators:

• Expert consultation on experiment design and specimen preparation 
• Training
• Imaging for a fee
• Ongoing assistance

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