Instrumentation

• Leica DMi8 inverted microscope with X-Y motorized stage
• Metal halide fluorescence lamp & halogen transmission lamp
• 10X, 20X Oil, 25X Water, 63X Water DIC & 63X Oil DIC objectives
• Laser Excitation for PA-GFP, DAPI, CFP, YFP, FITC, Rhodamine, & Cy5-like fluorophores
• Tunable & Pulsed White Light Laser: 470 - 670 nm
• Two additional lasers: 405 nm laser diode and a multi-argon (458, 477, 488, 514 nm lines)
• Six detectors: 2 Hybrid Spectral Detectors* (HyD); 2 Spectral SMD** HyDs, 1 standard Spectral PMT & 1 transmitted PMT (external)
• Galvo Z-stage
• Resonance scanner
• Pico Harp 300 Fluorescence Lifetime Microscopy (FLIM) Module
• LASX acquisition software
• SymphoTime64 (PicoQuant) FLIM Software
• Huygens Deconvolution software
• Tokai Hit Stage Top Incubator for Live Cell Imaging

* HyDs are specialized detectors utilizing GAsP photocathodes & avalanche photo-diodes
**Single Molecule Detectors (SMD)

• Fixed specimens mounted on standard slide with #1.5 cover slips
• Live cell specimens (LabTekII #1.5 multi-well or 35 mm (#1.5) cover bottom)
• FLIM FRET (fixed or live-cell)
• FRET Acceptor Bleaching (fixed)
• FRET Sensitized Emission (live-cell)
• FRAP (live-cell)
• 3D acquisition (Z-Stacks) and Time Lapse imaging
• Multi-Spectral Unmixing
• Large area, multi-dimensional tile scans
• Super resolution via image restoration processing (~130nm)

• Zeiss AxioObserver inverted microscope with X-Y motorized stage
• Metal halide fluorescent lamp & halogen transmission lamp
• 10x, 20x, 40x oil, 63x oil & 63x water immersion objectives
• DIC available with 10x, 20x, 40x & 63x objectives
• Laser Excitation for PA-GFP, DAPI, CFP, YFP, FITC, Rhodamine, & Cy5-like fluorophores
• Four solid state lasers: 405 nm, 488 nm, 561 nm, 640 nm
• Channel and Spectral detection – GaAsP PMT detectors
• Simultaneous detection in 3 channels available in Channel mode
• Airyscan array detector for 1.7X increase in XY resolution
• Transmitted light detector
• Live-cell incubation system: temperature, humidity and CO2 control
• Zen Blue Image Acquisition software

• Fixed specimens mounted on standard slide with #1.5 cover slips
• Live cell specimens (LabTekII #1.5 multi-well or 35 mm (#1.5) cover bottom)
• FRET Acceptor Bleaching (fixed)
• FRET Sensitized Emission (live-cell)
• FRAP (live-cell)
• 3D acquisition (Z-Stacks) and Time Lapse imaging
• Multi-Spectral Unmixing
• Large area, multi-dimensional tile scans
• Super resolution via image restoration processing (~130nm)

• Custom Olympus IX83 inverted microscope with ASI motorized XY stage with piezo Z insert
• Excelitas Turbo multi-spectral LED (wide field) and Lumencor white LED (bright field)
• 20x/0.8NA oil, 60x/1.5NA oil TIRF, 60x/1.2NA water, and 100x/1.4NA oil objectives
• DIC capable for 60x and 100x optics
• Olympus Real Time Controller for temporally accurate camera and excitation synchronization
• 4 laser line MITICO TIRF illuminator for independent pentration depths
• 4 individual laser lines for TIRF: 405nm (100mW); 488nm (100mW); 561nm (100mW) and 640nm (140mW)
• Direct Stochastic Optical Reconstruction Microscopy (dSTORM), Photoactivated Localization Microscopy (PALM) & Highly Inclined Laminar Optical (HILO) sheets imaging possible
• Hamamatsu Fusion sCMOS camera and Geminini W-View image splitter for 2 color simultaneous live cell acquisitons
• Non-photodestructive IR ZDC2 autofocus for extremely fast one shot or continuous autofocus capabilites
• BiopTechs objective heater for short-term live cell applications
• Olympus cellSens Dimension image acquisition software

• Fixed specimens with #1.5H cover slips
• Live cell specimens (LabTekII #1.5H multi-well or 35 mm (#1.5H) cover bottom)
• 3D acquisition (Z-Stacks) and Time Lapse imaging
• Specific applications include live-cell TIRF single molecule detection, HILO, 2D dSTORM or PALM
• GPU enabled post-processing provided by Huygens Localizer

• Inverted Olympus IX-71 inverted microscope
• Mercury fluorescence lamp and halogen transmission lamp
• Monochromator – 350nm-670nm excitation range
• Lasers: 405nm, 488nm,520nm and 642nm
• 20x, 60x H2O, 100x Oil, 150x Oil TIRF objectives
• Fluorescence filters for Quantum Dots, DAPI, FITC, & Rhod-like fluorophores, Fura-2, Indo-1
• Andor IQ Image Acquisition software
• Objective heater and Pathology Devices live cell incubation system w/ CO2, heat, humidity
• XYZ motorized stage
• Andor iXon EM-CCD camera
• Opti-split (dual) Image splitter

• Fixed specimen mounted on slides
• Live cell imaging with objective warmer
• Single Particle Tracking
• Total Internal Reflection Fluorescence (TIRF)
• Ratio-metric Imaging (dual excitation/single emission or single excitation dual emission)
• Live cells in culture
• Widefield Fluorescence – many fluorophores possible with monochromator
• Z-stacks & time series

• AxioPlanMOT upright microscope
• Metal Halide fluorescence lamp & halogen transmission lamp
• 10x, 20x, 40x Oil & 63x Oil objectives
• DAPI, FITC, Rhodamine, Cy5 long pass filters for use with Nuance camera. o Nuance spectral camera and software
• Motorized nosepiece, Z-stage, filter turret & shutter for easy operation.

• Fixed specimen mounted on slides
• Widefield fluorescence – specimen labeled with DAPI, FITC, Rhod, & Cy5-like fluorophores
• Brightfield – H&E, DAB, Giemsa, etc.
• Nuance spectral camera can be used to separate closely overlapping fluorophores, histochemical stains, distinguish autofluorescence and quantify labeling

• Zeiss AxioObserver inverted fluorescence microscope
• Hamamatsu ORCA Flash4.0v2 sCMOS monochrome camera (100fps at full resolution 2048x2048)
• Metal halide fluorescent lamp & halogen transmission lamp
• 10x, 20x, 63xDIC Oil, & 100x Oil objectives
• Phase Contrast for 10x and 20x objectives
• Fluorescence filter sets include: DAPI, FITC, Rhodamine/TRITC/Cy3, Texas Red & Cy5
• DIC available with 63x objectives
• Objective heater (63x water objective) o Motorized nosepiece for Z-stacking
• Motorized X-Y stage for multi-position image acquisition (fixed or live-cell time lapse imaging)
• SlideBook6 image acquisition software

• Fixed specimen mounted on slides
• Live cells in coverslip dish/heating with objective heater
• DIC (non-confocal) with 63x objectives, merged with fluorescence or alone
• Z-stacks, montage, and time series imaging

• AxioSkop 2 MOT upright microscope
• 4x, 10x, 20x, 40x, 40x Oil & 63x Oil
• Phase Contrast for 10x and 20x objectives
• AxioCam HR digital color camera
• Zeiss AxioVision image acquisition software
• Motorized Z-stage

• Fixed specimen mounted on slides for general histopathology
• Brightfield – H&E, DAB, Giemsa, etc.

• Nikon TE2000 inverted microscope
• XCITE120LED illumination source for fluorescence applications. Ideal for hit and run quality control in tissue culture fluorescence constructs
• Halogen transmission lamp
• 10x, 20xDIC, 40xDIC Oil, 60xDIC Oil objectives
• DIC available with all objectives except the 10x
• Fluorescence filters for DAPI, CFP, YFP, FITC, Rhodamine-like & Cy5 fluorophores
• Q-Imaging Retiga R3 monochrome camera and Ocular image acquisition software for basic image documentation.

• Fixed specimen mounted on slides
• Live cells in culture – dishes, plates, and flasks.
• Widefield Fluorescence – specimen labeled with DAPI, CFP, YFP, FITC, Rhod-like & Cy5 dyes
• DIC 20-60X, merged with fluorescence or alone

Software

• Huygens Deconvolution Scientific Volume Imaging (1)
• SlideBook (1)
• StereoInvestigator (1)
• Neurolucida (1)
• LASX (Leica confocal) (1)
• IQTL (Typhoon scanner analysis) (1)
• Zen (2)  
• LSM510 (2)
• Nuance spectral imaging software (2)

Analysis Options

• Co-localization
• Deconvolution
• Spectral unmixing
• Intensity analysis
• Morphological analysis
• 3D rendering
• Object counting
• Particle Tracking
• Preparation for publication

Typhoon FLA 7000 is a fast and versatile laser scanner for biomolecular imaging applications including sensitive and quantitative measurements of radioisotopic labels, chemifluorescent Western blots, and single fluorescence (visible excitation), as well as documentation of colorimetric stains (e.g., Coomassie blue and silver-stained gels).

Li-Cor Odessey Fc is a near infra-red scanner (680nm & 740nm) for biomolecular imaging applications including sensitive and quantitative measurements of chemiluminescent Western blots as well as documentation of colorimetric stains (e.g., Coomassie blue and silver-stained gels).