Acknowlegment
To acknowledge use of this shared resource, please include the following in your publications: This research was partially supported by UNM Comprehensive Cancer Center Support Grant NCI P30CA118100 and made use of the Fluorescence Microscopy and Cell Imaging shared resource.
Zeiss AxioObserver Microscope with Hamamatsu Flash4.0v2 sCMOS Camera Location: CRF212
Microscope Details
Zeiss AxioObserver inverted fluorescence microscope
Hamamatsu ORCA Flash4.0v2 sCMOS monochrome camera (100fps at full resolution 2048x2048)
Metal halide fluorescent lamp & halogen transmission lamp
10x, 20x, 63xDIC Oil, & 100x Oil objectives
Phase Contrast for 10x and 20x objectives
Fluorescence filter sets include: DAPI, FITC, Rhodamine/TRITC/Cy3, Texas Red & Cy5
DIC available with 63x objectives
Objective heater (63x water objective) o Motorized nosepiece for Z-stacking
Motorized X-Y stage for multi-position image acquisition (fixed or live-cell time lapse imaging)
SlideBook6 image acquisition software
Possible Applications
Fixed specimen mounted on slides
Live cells in coverslip dish/heating with objective heater
DIC (non-confocal) with 63x objectives, merged with fluorescence or alone
Z-stacks, montage, and time series imaging
Olympus DSU Spinning Disk Confocal/Stereology System Location: CRF212
Microscope Details
Olympus IX-81 inverted microscope
Metal halide fluorescence lamp and halogen transmission lamp
4x, 10x, 20x, 20x Oil, 40x, 60x Oil DIC, 60x Water DIC, 100x Oil objectives
X,Y, Z motorized stage
Pathology Devices live cell incubation system w/ CO2, heat, humidity
Color digital camera
EM CCD high sensitivity monochrome camera
Stereo Investigator software for design based stereology
Neurolucida software for neuron tracing
Possible Applications
Fixed specimen mounted on slides
Live cell imaging
Confocal and widefield fluorescence o Stereology
X,Y positioning, Z-stacks, time series, and montage imaging
DIC with both 60x objectives
Brightfield – H&E, DAB, Giemsa, etc
Darkfield
Olympus IX71 microscope with Andor iXon 897 EMCCD Camera Location: CRF218
Microscope Details
Inverted Olympus IX-71 inverted microscope
Mercury fluorescence lamp and halogen transmission lamp
Monochromator – 350nm-670nm excitation range
Lasers: 405nm, 488nm,520NM and 642nm
20x, 60x H2O, 100x Oil, 150x Oil TIRF objectives
Fluorescence filters for Quantum Dots, DAPI, FITC, & Rhod-like fluorophores, Fura-2, Indo-1
Andor IQ Image Acquisition software
Objective heater and Pathology Devices live cell incubation system w/ CO2, heat, humidity
XYZ motorized stage
Andor iXon EM-CCD camera
Opti-split (dual) Image splitter
Possible Applications
Fixed specimen mounted on slides
Live cell imaging with objective warmer
Single Particle Tracking
Total Internal Reflection Fluorescence (TIRF)
Ratio-metric Imaging (dual excitation/single emission or single excitation dual emission)
Live cells in culture
Widefield Fluorescence – many fluorophores possible with monochromator
Z-stacks & time series
Zeiss AxioPlan2 Microscope with Nuance Multi-Spectral Camera Location: CRF224
Microscope Details
AxioPlanMOT upright microscope
Metal Halide fluorescence lamp & halogen transmission lamp
10x, 20x, 40x Oil & 63x Oil objectives
DAPI, FITC, Rhodamine, Cy5 long pass filters for use with Nuance camera. o Nuance spectral camera and software
Motorized nosepiece, Z-stage, filter turret & shutter for easy operation.
Possible Applications
Fixed specimen mounted on slides
Widefield fluorescence – specimen labeled with DAPI, FITC, Rhod, & Cy5-like fluorophores
Brightfield – H&E, DAB, Giemsa, etc.
Nuance spectral camera can be used to separate closely overlapping fluorophores, histochemical stains, distinguish autofluorescence and quantify labeling
Zeiss Axioskop Microscope with AxioCam HR Color Camera Location: CRF226
Microscope Details
AxioSkop 2 MOT upright microscope
4x, 10x, 20x, 40x, 40x Oil & 63x Oil
Phase Contrast for 10x and 20x objectives
AxioCam HR digital color camera
Zeiss AxioVision image acquisition software
Motorized Z-stage
Possible Applications
Fixed specimen mounted on slides for general histopathology
Brightfield – H&E, DAB, Giemsa, etc.
Nikon TE2000 Microscope with Q-Imaging Retiga R3 Camera (monochrome)Location: CRF216
Microscope Details
Nikon TE2000 inverted microscope
XCITE120LED illumination source for fluorescence applications. Ideal for hit and run quality control in tissue culture fluorescence constructs
Halogen transmission lamp
10x, 20xDIC, 40xDIC Oil, 60xDIC Oil objectives
DIC available with all objectives except the 10x
Fluorescence filters for DAPI, CFP, YFP, FITC, Rhodamine-like & Cy5 fluorophores
Q-Imaging Retiga R3 monochrome camera and Ocular image acquisition software for basic image documentation.
Possible Applications
Fixed specimen mounted on slides
Live cells in culture – dishes, plates, and flasks.
Widefield Fluorescence – specimen labeled with DAPI, CFP, YFP, FITC, Rhod-like & Cy5 dyes
DIC 20-60X, merged with fluorescence or alone
Image Analysis Workstations Locations: CRF224 & CRF226
Workstation Details
Software
Huygens Deconvolution Scientific Volume Imaging (1)
SlideBook (1)
StereoInvestigator (1)
Neurolucida (1)
LASX (Leica confocal) (1)
IQTL (Typhoon scanner analysis) (1)
Zen (2)
LSM510 (2)
Nuance spectral imaging software (2)
Analysis Options
Co-localization
Deconvolution
Spectral unmixing
Intensity analysis
Morphological analysis
3D rendering
Object counting
Particle Tracking
Preparation for publication
GE Typhoon FLA 7000 Scanner Locations: CRF214
Scanner Details:
Typhoon FLA 7000 is a fast and versatile laser scanner for biomolecular imaging applications including sensitive and quantitative measurements of radioisotopic labels, chemifluorescent Western blots, and single fluorescence (visible excitation), as well as documentation of colorimetric stains (e.g., Coomassie blue and silver-stained gels).
Li-Cor Odessey FC Locations: CRF214
Scanner Details:
Li-Cor Odessey Fc is a near infra-red scanner (680nm & 740nm) for biomolecular imaging applications including sensitive and quantitative measurements of chemiluminescent Western blots as well as documentation of colorimetric stains (e.g., Coomassie blue and silver-stained gels).
