For further information on any of the techniques offered by the HTR-TASR or to set up a feasibility consultation, contact Cathy Martinez at firstname.lastname@example.org.
To acknowledge use of this shared resource, please include the following in your publications: This research was partially supported by UNM Comprehensive Cancer Center Support Grant NCI P30CA118100 and made use of the Human Tissue Repository and Tissue Analysis Shared Resource, which receives additional support from The University of New Mexico School of Medicine Department of Pathology.
A. This comprises cutting sections of frozen or paraffin-embedded formalin fixed tissues. B. The tissue can be stained with an H&E stain or remain unstained.
The laboratory can stain tissue sections with standard antibodies using protocols already in place. Alternatively, the laboratory can work up antibodies using the VENTANA DISCOVERY platform that allows for multiple staining combinations to optimize staining results.
Fluorescence-tagged antibodies can also be used to detect your protein of interest.
Tissue microarray design and construction: This entails collecting donor paraffin blocks, selecting the areas of interest to core, and placing the cores in a blank recipient paraffin block. The advantages are in speed and cost, as multiple samples may be assessed on one slide. The typical TMA contains approximately 30-50 cores of 1 -2.0 mm diameter. Approximately 50 to 200 sections may be obtained from one TMA block.
The HTR-TASR personnel can retrieve archival tissue blocks from UNM Hospital.
This usually comprises collection of excess or remnant tissues left over at the time of surgical resection after diagnostic samples have been taken for surgical pathologist microscopic review and enough specimen is retained in paraffin for potential future testing for patient care. HTR-TASR personnel will collect the tissues in the Gross Dissection Room of UNM HSC Surgical Pathology. Currently, the HTR does not perform venipunctures but will bank such specimens as requested by researchers.
HTR-TASR project consultants can collect pathology and clinical data that are stripped of HIPAA identifiers while coded to matching samples.
1. DNA and RNA isolations from multiple types of specimens (e.g., fresh/frozen blood, transformed cells, frozen tissue, paraffin embedded tissue); including quantitation and quality assessment of the RNA.
2. Polymerase Chain Reaction (PCR) amplification from DNA or RNA targets, including primer design, PCR optimization, set up PCR and RT-PCR, and gel electrophoresis for quality assurance.
3. Gene expression quantitation (real time PCR): including primer design.
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