To acknowledge use of this shared resource, please include the following in your publications: This research was partially supported by UNM Comprehensive Cancer Center Support Grant NCI P30CA118100 and made use of the Human Tissue Repository and Tissue Analysis Shared Resource, which receives additional support from The University of New Mexico School of Medicine Department of Pathology.
Images of H&E or immunohistochemically stained tissue sections or immunofluorescence sections are captured on the Leica Versa 200 digital scanner and uploaded to the HALO imaging and annotation software.
The HALO software allows you to annotate the tissue (tumor, stroma, muscle, fat etc.) and then count cells that are positive for protein of choice (Figure) within each of these annotated regions. It will also enable you to analyze and quantify nearest neighbor interactions.
The software includes a figure-making tool that can create multi-panel photos of images in publishable formats including reference frames and scale bars.