July 5, 2016
By UNM CCC 
M. Fero 1/8/09 Procedure Register online Bring mouse in clean cage to imager Sign in on log sheet Clean inside of Xenogen system and external mouse anesthesia chamber. To minimize background, use black plastic background sheet for fluorescence, use black paper for luminescence. Anesthesia Turn on gas cylinder (but don’t change gas regulator on cylinder) […]
Tags: Fero-Mice
July 5, 2016
By UNM CCC
What is the MPD? The MPD is a laboratory relational database that allows users to cross-reference mouse inventory data, breeding records, pedigrees, pathology data, histology images, autoradiographs, PCR genotyping protocols, freezer archives, and reagents (specifically plasmids, oligonucleotides, and antibodies). It is comprised of a set of FileMaker templates which can be used on a single […]
Tags: Fero-Mice
June 28, 2016
By UNM CCC
(M.Fero 10/2013) Reagents Lysis Buffer: (10 mM Tris pH8, 100 mM NaCl, 25 mM EDTA, 0.5%SDS), store at room temp. 10 mg/mL Proteinase K: 100 mg (Roche) + 10 mL buffer (10 mM Tris pH8.0, 20 mM CaCl2, 50% (v/v) glycerol), store at -20ºC in 1.5 mL aliquots. Lysis buffer + proteinase K (1 mg/mL): […]
Tags: Fero-Mice
June 28, 2016
Mouse p27 PCR Genotyping Using Gitschier Buffer
By UNM CCC
(PCR Recipe: Kogan et al, New Engl J Med 317: 985-990) Primer Sequences p27KO mice. JAX Stock 2781 (C57BL/6), JAX Stock 3122 (129S4) Fero ML, et al. Cell. (1996) 85:733-44 KO (knockout allele; Contains Neo) Neo-1 (CCTTCTATCGCCTTCTTG) K-3 (TGGAACCCTGTGCCATCTCTAT) Size: KO(-) = 0.5 kB WT (wildtype allele) K-3 (above) K-5 (GAGCAGACGCCCAAGAAGC) Size: WT(+) = 1 […]
Tags: Fero-Mice
June 28, 2016
By UNM CCC
M. Fero — 4/11/00, updated 4/25/03 Materials Plastic tubes with anticoagulant: PBS + 2 µL anticoagulant for each 100 µL blood to be collected Anticoagulant: 10% Na2EDTA, pH=7.4. 250 µL of fresh mouse blood in plastic tubes containing EDTA. RBC lysis buffer (388 mM NH4Cl, 29.7 mM NaHCO3, 25 µM Na2EDTA) 20.75 g. NH4Cl 2.5 […]
Tags: Fero-Mice
